Uji Aktivitas Antibakteri dan Formulasi Gel Masker Peel-Off dari Ekstrak Etanol Daun Sirih Merah (Piper crocatum)
DOI:
https://doi.org/10.33143/jhtm.v7i1.1956Abstract
Banyak masyarakat yang menggunakan krim atau perawatan wajah yang beredar dipasaran dan secara illegal. Ekstrak daun sirih merah (Piper crocatum) mengandung senyawa saponin dan tanin yang memiliki aktivitas dalam menghambat bakteri Staphylococcus aureus, dan kosmetik yang terbuat dari herbal lebih aman digunakan. Untuk mengetahui aktivitas antibakteri dari daun sirih merah (P. crocatum) dan membuat formulasi gel peel-off. Penelitian ini dilakukan secara eksperimental dengan menguji aktivitas antibakteri dan membuat suatu formulasi. Ekstraksi daun sirih merah (P. crocatum) menggunakan etanol 96% dengan metode maserasi. Untuk uji aktivitas antibakteri dibuat beberapa konsentrasi yaitu 200 mg/ml, 100 mg/ml, 50 mg/ml, 40 mg/ml, 30 mg/ml, 20 mg/ml, 10 mg/ml dan untuk formulasi dengan konsentrasi 2,5 % dan 5%. Hasil pengujian menunjukkan bahwa ekstrak etanol daun sirih merah memiliki aktivitas antibakteri terhadap Staph. aureus yang ditandai dengan adanya zona hambat. Zona hambat tertinggi terhadap Staph. aureus yaitu ditunjukan oleh ekstrak dengan konsentrasi 200 mg/ml dengan diameter zona hambat 19,5 mm dan zona hambat terendah pada konsentrasi 10 mg/ml dengan diameter zona hambat 4 mm. Untuk formulasi pada konsentrasi 2,5 % dan 5 % sudah memiliki pH yang sesuai dengan kulit dan pada konsentrasi 5% memiliki daya mengering yang terlalu lama. ekstrak etanol daun sirih merah (P. crocatum) memiliki aktivitas antibakteri karakteristik gel sudah memenuhi syarat.
Kata Kunci : Ektrak etanol daun sirih merah, aktivitas antibakteri, Staphylococcus aureus, masker gel peel-off
Many teenagers are experienced They often use cosmetics face cream aside from illegal cosmetics. Betel leaf extract (Piper crocatum) contains saponin and tannin compounds that can inhibit the growth of Staphylococcus aureus bacteria. In addition, cosmetics that made from herbs are more safer to be used. This study aimed to determine antibacterial activity of the betel leaves and antiacne formulation. This research was conducted experimentally by examine antibacterial activity and create a formulation. Extraction of red betel leaf (P. crocatum) using 96% ethanol by maceration method. The extract which is acquired from the procedure is used to analyze antibacterial activity and make formulations for peel- off gel masks. To test the antibacterial activity several concentrations were made, viz. 200 mg / ml, 100 mg / ml, 50 mg / ml, 40 mg / ml, 30 mg / ml, 20 mg / ml, 10 mg / ml, and two concentration viz. 2.5 % and 5% for gel mask formulations. The results showed that the ethanol extract of the betel leaves could inhibit the growth of Staph. aureus bacteria which was characterized by the presence of inhibitory zones. The highest inhibition zone against this bacteria was observed on extract concentration 200 mg/ml i.e 19.5 mm in diameter, while the lowest inhibition zone was shown by concentration 10 mg/ml i.e 4 mm in diameter. Each formulation at the concentration of 2.5% and 5% contained a pH that matches on the skin. However, the formulation at the concentration of 5% recorded a long drying power. It can be concluded that the ethanol extract of the betel leaves (P.crocatum) has an antibacterial activity because it can inhibit the growth of the Staph. aureus bacteria.
Keywords: Ethanol extract of betel leaves, antibacterial activity, Staphylococcus aureus, peel-off gel mask.
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