Pengaruh Ekstrak Etanol Bit Merah (Beta vulgaris L.) terhadap Jumlah Arteriole Endometrium pada Tikus (Rattus norvegicus) yang dipapar Asap Rokok
DOI:
https://doi.org/10.33143/jhtm.v9i1.2810Abstract
Paparan asap rokok sering terjadi dikalangan masyarakat, ini dapat menyebabkan terjadinya peningkatan ROS sehingga antioksidan dalam tubuh menurun. ROS yang meningkat akan menyebabkan gangguan pada organ reproduksi seperti endometrium dan ovarium. Akibat gangguan ini maka akan berpengaruh pada tingkat kesuburan (infertilitas) pada wanita. Bit merah mengandung senyawa betalain yang tinggi akan antioksidan yang dapat menetralisir ROS yang ada didalam tubuh yang dapat mengganggu organ reproduksi wanita yang dapat menyebabkan salah penyebab ketidaksuburan pada wanita. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol bit merah (Beta vulgaris L.) dengan berbagai dosis terhadap jumlah arteriole pada endometrium tikus yang dipapar asap rokok. Jenis penelitian ini adalah true experiments dengan rancangan post test only control group design. Menggunakan 25 ekor tikus betina yang dibagi menjadi 5 kelompok yaitu kelompok kontrol negatif (tanpa perlakuan), kelompok kontrol positif (tikus yang dipapar asap rokok 2 batang/hari) selama 56 hari, kelompok perlakuan I (dipapar asap rokok 2 batang/hari dan diberi ekstrak etanol bit merah dengan dosis 125 mg/kgBB/hari) selama 56 hari, perlakuan II (dipapar asap rokok 2 batang/hari dan diberi ekstrak etanol bit merah dengan dosis 250 mg/kgBB/hari) selama 56 hari dan perlakuan III (dipapar asap rokok 2 batang/hari dan diberi ekstrak etanol bit merah dengan dosis 500 mg/kgBB/hari) selama 56 hari. Metode yang digunakan untuk melihat jumlah arteriole pada endometrium tikus dengan menggunakan pewarnaan Haematoksilin Eosin. Kemudian masing-masing slide diamati dibawah mikroskop Manual Dot Slide Olympus XC 10 yang dilihat per lapang pandang. Data dianalisis dengan menggunakan One Way Anova, hasil nilai p-value jumlah arteriole (p=0.000). Hasil penelitian membuktikan bahwa pemberian ekstrak etanol bit merah dengan dosis 500 mg/kgBB/hari dapat meningkatkan jumlah arteriole pada endometrium tikus yang dipapar asap rokok. Penelitian ini membuktikan bahwa pemberian ekstrak etanol bit merah dapat meningkatkan jumlah arteriole pada endometrium tikus yang dipapar asap rokok. Kesimpulan pada penelitian ini adalah terjadi peningkatan jumlah arteriole pada kelompok yang diberi ekstrak etanol bit merah dengan berbagai dosis. Tetapi dosis ekstrak etanol bit merah yang dianggap paling cepat meningkatkan jumlah arteriole adalah kelompok perlakuan III dengan dosis 500 mg/kgBB/hari.
Kata Kunci: Jumlah Arteriole, Rattus norvegicus, Bit Merah (Beta vulgaris L.)
Exposure to cigarette smoke often occurs among the community, this can cause an increase in ROS so that antioxidants in the body decrease. Increased ROS will cause disturbances in reproductive organs such as the endometrium and ovaries. As a result of this disorder it will affect the level of fertility (infertility) in women. Red beets contain betalain compounds which are high in antioxidants which can neutralize ROS in the body which can interfere with the female reproductive organs which can cause one of the causes of infertility in women. This study aims to determine the effect of red beet (Beta vulgaris L.) ethanol extract with various doses on the number of arterioles in the endometrium of rats exposed to cigarette smoke. This type of research is true experiments with a post test only control group design. Using 25 female rats divided into 5 groups namely negative control group (no treatment), positive control group (rats exposed to cigarette smoke 2 cigarettes/day) for 56 days, treatment group I (exposed to cigarette smoke 2 cigarettes/day and given red beet ethanol extract at a dose of 125 mg/kgBW/day) for 56 days, II treatment (exposed to cigarette smoke 2 sticks/day and given red beet ethanol extract at a dose of 250 mg/kgBW/day) for 56 days and treatment III (exposed to cigarette smoke 2 sticks/day and given red beet ethanol extract at a dose of 500 mg/kg/day) for 56 days. The method used to see the number of arterioles in the rat endometrium using Haematoxylin Eosin staining. Then each slide was observed under a Manual Dot Slide Olympus XC 10 microscope which was viewed per field of view. Data were analyzed using One Way Anova, the results of the p-value of the number of arterioles (p=0.000). The results of the study proved that administration of red beetroot ethanol extract at a dose of 500 mg/kgBW/day could increase the number of arterioles in the endometrium of rats exposed to cigarette smoke. This study proves that administration of red beetroot ethanol extract can increase the number of arterioles in the endometrium of rats exposed to cigarette smoke. The conclusion in this study was that there was an increase in the number of arterioles in the group given red beet ethanol extract at various doses. But the dose of red beet ethanol extract that was considered the fastest to increase the number of arterioles was the treatment group III at a dose of 500 mg/kg/day.
Keywords: Number of Arterioles, Rattus norvegicus, Red Beet (Beta vulgaris L.)
References
Clifford, T., Howatson, G., West, J.D., Stevenson, J.E. 2015. ‘The potential benefits of red beetroot supplementation in health and disease’, Nutrients, 7(4), pp. 2801–2822. doi: 10.3390/nu7042801.
Dechanet, C., Anahory, T., Daude M.C.J., Quantin. X., Reyftmann. L., Hamamah., et al. 2010. Effects of cigarette smoking on reproduction’, Human Reproduction Update, Vol.17, No.1 pp. 76–95, 2011 Advanced Access publication on August 4, 2010 doi:10.1093/humupd/dmq033.
Deka, K.P., Sarma, S. 2010. Psychological aspects of infertility. British Journal of Medical Practitioners, September 2010, Volume 3, Number 3.
Frisca, C.T., Sarjhono dan Sandra, F. 2009. Angiogenesis : patofisiologi dan aplikasi klinis. JKM. 8 (2) : 174-187
Kawashima, A., Koide, K., Ventura, W., Hori, K., Takenaka, S., Maruyama, D., Matsuoka, R., Ichizuka, K., Sekizawa, A. 2014. Effects of maternal smoking on the placental expression of genes related to angiogenesis and apoptosis during the first trimester. http://sci-hub.tw/https://doi.org/10.1371/journal.pone.0106140
Mroczek, A., Kapusta, I., Janda, B., Janiszowska, W. 2012. ‘Triterpene saponin content in the roots of red beet (Beta vulgaris L.) cultivars’, Journal of Agricultural and Food Chemistry, 60(50), pp. 12397–12402. doi: 10.1021/jf303952x.
Soares, R.S and Melo, A.M. 2008. Cigarette smoking and reproductive function. Instituto Valenciano de Infertilidad, IVI-Lisboa, Lisbon, Portugal and Instituto Valenciano de Infertilidad, Instituto Universitario IVI, Valencia, Spain
Downloads
Issue
Section
License
I. ORIGINALITY, AUTHORSHIP, AND COPYRIGHT
The manuscripts submitted for publication must be research works written in English that have not been previously published and  are not being considered for publication elsewhere. All the authors must have agreed to the submission and to the order of their names on the title page. They must also have agreed that the corresponding author may act on their behalf throughout the editorial review and publication process. The corresponding author is responsible for obtaining such agreement.
Requests for changes in authorship (order of listing or addition or deletion of names) after submission should be accompanied by signed statements of agreement from all the parties involved. To maintain and protect the publisher‘s ownership and rights and to protect the original authors from misappropriations of their published work, author(s) are required to sign a Journal Publishing Agreement and send it to the editorial office (jhtm@uui.ac.id).
II. PERMISSION
The author is responsible for acquiring the permission(s) to reproduce any copyrighted figures, tables, data, or text that are being used in the submitted paper. Authors should note that text quotations of more than 250 words from a published or copyrighted work will require grant of permission from the original publisher to reprint. The written permission letter(s) must be submitted together with the manuscript.
III. EDITORIAL REVIEW AND REVISION
All papers will be critically read by at least two anonymous reviewers, selected for their competence in the subject area of the paper. Acceptance of the paper will depend upon its scientific merit and suitability for the journal. A paper may be accepted in its original form or subject to revision.
The reviewers' (and editor's) suggestions will be conveyed to the author, who will then have an opportunity to revise the paper. A manuscript returned to an author for revision can be held for a maximum of eight weeks.
The accepted paper will be edited by native English speakers. The cost of language editing service is currently free.
IV. PROOFS
Galley proofs (PDF format) for an accepted article will be sent by e-mail to the corresponding author for copyediting corrections. The core content of an article cannot be changed at this time. It is the author's responsibility to check the entire manuscript, including tables, figure legends, and cited reference numbers, not just the items queried. The proofs should be corrected and mailed to the editorial office within 48 hours of receipt in order to expedite publication. Only one set of corrections will be accepted. In other words, it is important to ensure that all of your corrections are sent to us in one communication.
V. Â REPRINTS
Five free reprint copies of the published article will be shipped to the corresponding authors in Indonesia only, approximately two weeks after the issue is printed. Authors, including overseas authors, who wish to purchase reprints, are required to complete the Reprint Order Form, which will be sent with the galley proofs to corresponding authors. This form must be returned together with the corrected galley proofs.
