Uji Efektivitas Antibakteri Minyak Atsiri Kulit Jeruk Manis (Citrus sinensis) dan Batang Serai (Cymbopogon citratus) Terhadap Bakteri Escherichia coli
DOI:
https://doi.org/10.33143/jhtm.v10i1.4034Abstract
Diare di Indonesia merupakan suatu penyakit yang umum terjadi dan dapat menjadi situasi Kejadian Luar Biasa (KLB) yang potensial sering disertai dengan kematian. Diare dapat dipicu oleh berbagai jenis bakteri, salah satunya adalah bakteri Escherichia coli, bakteri ini biasanya hidup disaluran pencernaan makhluk hidup. Metode pada penelitian ini menggunakan metode difusi cakram Kirby Bauer dengan beberapa konsentrasi minyak atsiri kulit jeruk manis dan batang serai yaitu, 15%, 20%, 25% dan 30%. Ceftiaxone 5% sebagai kontrol positif dan n-Hexan 99% sebagai kontrol negatif. Dari hasil penelitian menunjukkan bahwa, zona hambat pada minyak atsiri kulit jeruk manis pada konsentrasi 15%, 20%, 25% dan 30% memiliki masing-masing zona hambat dengan rata-rata 6,27 mm, 6,33 mm, 6,35 mm, 7,26 mm. Zona hambat pada minyak atsiri batang serai dengan konsentrasi yang sama memiliki masing-masing zona hambat dengan rata-rata 6,21 mm, 6,24 mm, 6,32 mm dan 7,2 mm. Sedangkan pada kombinasi minyak atsiri kulit jeruk manis dan batang serai tidak menghasilkan zona hambat (0 mm).
Kata kunci: Kulit jeruk manis, batang serai, aktivitas, Escherichia coli
Diarrhea in Indonesia is a common disease and can be a potential Extraordinary Event (KLB) situation that is often accompanied by death. Diarrhea can be triggered by various types of bacteria, one of which is the Escherichia coli bacteria, this bacteria usually lives in the digestive tract of living things. The method in this study uses the Kirby Bauer disc diffusion method with several concentrations of essential oils of sweet orange peel and lemongrass stems, namely, 15%, 20%, 25% and 30%. Ceftiaxone 5% as a positive control and n-Hexan 99% as a negative control. The results of the study showed that the inhibition zones in sweet orange peel essential oil at concentrations of 15%, 20%, 25% and 30% had each inhibition zone with an average of 6.27 mm, 6.33 mm, 6.35 mm, 7.26 mm. The inhibition zones in lemongrass stem essential oil with the same concentration had each inhibition zone with an average of 6.21 mm, 6.24 mm, 6.32 mm and 7.2 mm. Meanwhile, the combination of essential oils, sweet orange peel and lemongrass stems does not produce an inhibition zone (0 mm).
Keywords: Sweet orange peel, lemongrass stalks, activity, Escherichia coliReferences
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