UJI AKTIVITAS ANTIBAKTERI EKSTRAK BUNGA CENGKEH (Syzygium aromaticum L.) TERHADAP PERTUMBUHAN BAKTERI Salmonella
DOI:
https://doi.org/10.33143/jhtm.v10i2.4312Abstract
S. typhi merupakan bakteri bakteri Gram negatif yang dapat menimbulkan gejala demam tinggi yang lebih dari seminggu, konstipasi, nyeri abdomen, pusing, mual, muntah, kulit gatal dan timbul bercak berwarna kemerahan, bahkan kehilangan kesadaran. konsentrasi berapa kadar hambat minimum (KHM) ekstrak bunga cengkeh (Syzygium aromaticum L) mampu menghambat pertumbuhan bakteri S. typhi. Penelitian ini menggunakan metode difusi dan dilusi menggunakan kertas cakram dengan beberapa konsentrasi ekstrak etanol bunga cengkeh 10%, 50% dan 100%. Chloramfenikol 0,3% sebagai kontrol positif dan Dimetil sulfoksida (DMSO) 1% sebagai kontrol negatif. Data yang diperoleh dari hasil penelitian diolah dengan metode komputerisasi yaitu uji anova. Data hasil penelitian dianalisis menggunakan uji one way anova p=0,000. Hasil penelitian uji aktivitas antibakteri serta kadar hambat minimum (KHM) diperoleh ekstrak etanol bunga cengkeh konsentrasi 10%, 50%, dan 100% memiliki ratarata zona hambat 8,16 mm, 11,32 mm, 15,93 mm. Aktivitas antibakteri dengan konsentrasi terbesar 100% dengan rata-rata zona hambat 15,93 mm, dalam kategori kuat, terdapatnya perbedaan yang signifikan antara kelompok perlakuan dengan kontrol positif.
Kata kunci : Bunga Cengkeh, antibakteri, aktivitas, Salmonella thypi                  Â
S. typhi is a Gram-negative bacterium that can cause symptoms of high fever for more than a week, constipation, abdominal pain, dizziness, nausea, vomiting, itchy skin and reddish spots and even loss of consciousness. The aim of the research is to determine antibacterial activity and find out at what concentration the minimum inhibitory concentration (MIC) of clove flower extract (Syzygium aromaticum) is able to inhibit the growth of S. typhi bacteria.This research uses the diffusion and dilution method using paper discs with several concentrations of clove flower ethanol extract, 10%, 50% and 100%. Chloramphenicol 0.3% as a positive control and Dimethyl sulfoxide (DMSO) 1% as a negative control. The data obtained from the research results were processed using a computerized method, namely the anova test. The research data were analyzed using the one way anova test p=0.000. The results of the antibacterial activity test and minimum inhibitory content (MIC) showed that clove flower ethanol extract with concentrations of 10%, 50% and 100% had an average inhibition zone of 8.16 mm, 11.32 mm, 15.93 mm. Antibacterial activity with the greatest concentration of 100% with an average inhibition zone of 15.93 mm, in the strong category, there is a significant difference between the treatment group and the positive control.
Keywords: Clove flowers, antibacterial, activity, Salmonella typhi
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