AKTIVITAS ANTIBAKTERI SALEP EKSTRAK DAUN PUDING HITAM (Graptophyllum pictum L.) TERHADAP LUKA YANG TERINFEKSI BAKTERI Staphylococcus aureus PADA MENCIT (Mus musculus L.)
DOI:
https://doi.org/10.33143/jhtm.v8i1.4197Abstrak
Tanaman puding hitam (Graptophyllum pictum L.) merupakan tanaman liar yang sering ditemukan di daerah pedesaan atau sengaja ditanam sebagai tanaman obat yang mempunyai kandungan kimia seperti alkaloid, saponin, tanin, flavonoid dan steroid yang diketahui dapat mempercepat proses penyembuhan luka. Tujuan penelitian ini untuk mengetahui pengaruh salep Ekstrak Etanol Daun Puding Hitam (EEDPH) dan konsentrasi efektif terhadap penyembuhan luka infeksi bakteri Staphylococcus aureus. Metode yang digunakan ialah eksperimen laboratorim, pembuatan formulasi salep menggunakan ekstrak etanol daun puding hitam dengan konsentrasi 10%, 15% dan 20%. Hasil penelitian menunjukkan bahwa salep ekstrak etanol daun puding hitam dengan konsentrasi 10%, 15% dan 20% memenuhi syarat pengujian salep yang meliputi uji organoleptik, homogenitas, uji pH, daya sebar dan daya lekat. Hasil uji terhadap luka infeksi bakteri Staphylococcus aureus pada mencit (Mus musculus L.) menunjukkan bahwa salep (EEDPH) memiliki efek penyembuhan pada konsentrasi 20%. Hal ini dapat dilihat dari mengeringnya luka dan mengecilnya panjang luka pada mencit pada hari ke 6 sampai hari ke 14.Â
Graptophyllum pictum L. is a wild plant that is often found in rural areas or deliberately planted as medicinal plants that have chemical contents such as alkaloids, saponins, tannins, flavonoids and steroids which are known to accelerate the wound healing process. The purpose of this study was to determine the effect of ointment Graptophyllum pictum Leaf Ethanol Extract (EEDPH) and effective concentration on wound healing of Staphylococcus aureus infection. The method used is laboratory experiments, making ointment formulations using ethanol extracts of Graptophyllum pictum leaves with concentrations of 10%, 15% and 20%. The results showed that the ointment of Graptophyllum pictum leaves ethanol extract with a concentration of 10%, 15% and 20% met the conditions of the ointment test which included organoleptic test, homogeneity, pH test, spreadability and adhesion. Test results on Staphylococcus aureus bacterial wound infections in mice (Mus musculus L.) showed that the ointment (EEDPH) had a healing effect at a concentration of 20%. This can be seen from the drying of the wound and the reduced length of the wound in mice on the 6th day to the 14th day.
Referensi
Andayani R. Umi, Y dan Dina. M., 2015. Uji Efektivitas Ekstrak Daun Ungu (Graptophyllum pictum L.) Sebagai Penyembuhan Luka. Jurnal Prosiding Penelitian Spesia Unisba. Vol 5. No 5. Hal 311-315.
Andi M, I. 2015. Efektifitas Salep Ekstrak Daun Sirih (Piper betle L.) Terhadap Penyembuhan Luka Sayat Pada Ayam Petelur (Gallus leghorn).
Skripsi. Fakultas Kedokteran Universitas Hasanuddin Makasar.
Dalimartha S. 1999. Atlas Tumbuhan Obat Indonesia, jilid 1, Jakarta: Trubus Agriwidia.
Departemen Kesehatan Republik Indonesia. 1989. Sediaan Galenik Depkes RI. Jakarta.
Djumaati, F. Paulina, V. Yamlean Y dan Widya, AL. 2018. Formulasi Sediaan Salep ekstrak Etanol Daun Kelor (Moringa oleifera Lamk.) dan Uji Aktivitas Antibakterinya Terhadap Bakteri Staphylococcus aureus. Jurnal Ilmiah Farmasi UNSRAT Vol. 7 No.1 Hal 22-29.
Fauzi. D. 2016. Aktivitas Antibakteri Ekstrak Daun Ungu (Graptophyllum pictum L.) Terhadap Staphylococcus aureus dan Pseudomonas aeruginosa. Skripsi. Universitas Atma Jaya Yogyakarta Fakultas Teknologi, Program Studi Biologi Yogyakarta.
Lachman. 1994. Teori dan Praktek Farmasi Industri (Terjemahan) Jilid
II. Jakarta: Universitas Indonesia.
Marpaung M. P. Alwi, A dan witri. 2017. Karakterisasi Dan Skrining Fitokimia Ekstrak Kering Akar Kuning. (Fibraurea chloroleuca Miers). Jurnal Prosiding Seminar Nasional Kimia UNY FMIPA.
Parwanto, M.E., Senjaya, H. dan Hoseo,
J.E. 2013. Formulasi Salep Antibakteri Ekstrak Etanol Daun Tembelekan ( Lantana camar L.) Jurnal Ilmiah Farmasi. Vol. 2 No.3 Hal 104-108.
Paulina V,Y. Yamlean dan Widya A, L. 2016. Aktivitas Antibakteri Salep
Ekstrak Daun Kembang Sepatu (Hibicus rosa sinensis L.) Terhadap Luka Yang Terinfeksi Bakteri Staphylococcus aureus Pada Kelinci (Oryc tolaguscuniculus). Jurnal Ilmiah Farmasi. Vol. 5 No.4. Hal 298-304.
Rikomah,Yanti, Y. N., Juarsah, W. 2017. Uji Daya Hambat Ekstrak Etanol Daun Puding Hitam (Graptohyllum pictum L.). Pada Pertumbuhan Bakteri Pseudomonas aeruginosa Jurnal Sain dan Teknologi Farmasi. Vol.19 No.1 Hal 22-26.
Rikomah S, E. Elmitra. 2018. Formulasi Sediaan Krim Ekstrak Etanol Puding Hitam (Graptophyllum pictum L.) Jurnal Katalisator Vol.3 No.1 Hal.43-52.
Sari. A dan Amy. M. 2016. Formulasi Sediaan Salep Ekstrak Etanol Rimpang Kunyit (Curcurma longa Linn). Jurnal SEL. Vol.3 No.1 Hal 16-23.
Septiatin E., 2008. Apotek Hidup dari Rempah- Rempah Tanaman Hias dan Tanaman Liar, Bandung: CV YRAMA WIDYA.
Ummanah C. 2017. Uji Skrining Fitokimia Dan Antimikroba Ekstrak Daun Handeuleum (Graptophyllum pictum L.) Dalam Menghambat Pertumbuhan Mikroba Patogen. Skripsi. Universitas Medan Area Medan Fakultas Biologi.
Unduhan
Terbitan
Bagian
Lisensi
I. ORIGINALITY, AUTHORSHIP, AND COPYRIGHT
The manuscripts submitted for publication must be research works written in English that have not been previously published and  are not being considered for publication elsewhere. All the authors must have agreed to the submission and to the order of their names on the title page. They must also have agreed that the corresponding author may act on their behalf throughout the editorial review and publication process. The corresponding author is responsible for obtaining such agreement.
Requests for changes in authorship (order of listing or addition or deletion of names) after submission should be accompanied by signed statements of agreement from all the parties involved. To maintain and protect the publisher‘s ownership and rights and to protect the original authors from misappropriations of their published work, author(s) are required to sign a Journal Publishing Agreement and send it to the editorial office (jhtm@uui.ac.id).
II. PERMISSION
The author is responsible for acquiring the permission(s) to reproduce any copyrighted figures, tables, data, or text that are being used in the submitted paper. Authors should note that text quotations of more than 250 words from a published or copyrighted work will require grant of permission from the original publisher to reprint. The written permission letter(s) must be submitted together with the manuscript.
III. EDITORIAL REVIEW AND REVISION
All papers will be critically read by at least two anonymous reviewers, selected for their competence in the subject area of the paper. Acceptance of the paper will depend upon its scientific merit and suitability for the journal. A paper may be accepted in its original form or subject to revision.
The reviewers' (and editor's) suggestions will be conveyed to the author, who will then have an opportunity to revise the paper. A manuscript returned to an author for revision can be held for a maximum of eight weeks.
The accepted paper will be edited by native English speakers. The cost of language editing service is currently free.
IV. PROOFS
Galley proofs (PDF format) for an accepted article will be sent by e-mail to the corresponding author for copyediting corrections. The core content of an article cannot be changed at this time. It is the author's responsibility to check the entire manuscript, including tables, figure legends, and cited reference numbers, not just the items queried. The proofs should be corrected and mailed to the editorial office within 48 hours of receipt in order to expedite publication. Only one set of corrections will be accepted. In other words, it is important to ensure that all of your corrections are sent to us in one communication.
V. Â REPRINTS
Five free reprint copies of the published article will be shipped to the corresponding authors in Indonesia only, approximately two weeks after the issue is printed. Authors, including overseas authors, who wish to purchase reprints, are required to complete the Reprint Order Form, which will be sent with the galley proofs to corresponding authors. This form must be returned together with the corrected galley proofs.
