ISOLASI SENYAWA BIOAKTIF DARI EKSTRAK DAUN KELOR (Moringa oleifera) DAN POTENSINYA SEBAGAI ANTIOKSIDAN UNTUK FORTIFIKASI PANGAN FUNGSIONAL
DOI:
https://doi.org/10.33143/jhtm.v11i1.5216Kata Kunci:
Daun kelor (Moringa oleifera), Senyawa bioaktif, Antioksidan, Fortifikasi pangan Pangan fungsionalAbstrak
Abstrak
Penelitian ini bertujuan mengisolasi senyawa bioaktif dari daun kelor (Moringa oleifera) serta mengevaluasi potensi antioksidannya untuk fortifikasi pangan fungsional. Daun kelor diekstraksi dengan metode maserasi menggunakan etanol 70% dan dilanjutkan dengan fraksinasi pelarut n-heksana, etil asetat, dan metanol. Identifikasi senyawa bioaktif dilakukan dengan KLT, KCKT, spektrofotometri UV-Vis, dan FTIR, sedangkan uji aktivitas antioksidan dilakukan menggunakan metode DPPH. Hasil penelitian menunjukkan bahwa fraksi etil asetat memiliki aktivitas antioksidan paling tinggi dengan IC50 sebesar 48,72 µg/mL, yang dikategorikan sebagai antioksidan sangat kuat. Analisis fitokimia memperlihatkan bahwa senyawa utama yang berperan adalah flavonoid, fenolik, dan tanin. Temuan ini menegaskan bahwa daun kelor merupakan sumber antioksidan alami yang potensial dan dapat diaplikasikan sebagai bahan fortifikasi pangan fungsional untuk meningkatkan kualitas gizi serta memberikan manfaat protektif terhadap stres oksidatif.
Referensi
Anwar, F., Latif, S., Ashraf, M., & Gilani, A. H. (2007). Moringa oleifera: A food plant with multiple medicinal uses. Phytotherapy Research, 21(1), 17–25. https://doi.org/10.1002/ptr.2023
Fahey, J. W. (2005). Moringa oleifera: A review of the medical evidence for its nutritional, therapeutic, and prophylactic properties. Part 1. Trees for Life Journal, 1(5), 1–33.
Sreelatha, S., & Padma, P. R. (2009). Antioxidant activity and total phenolic content of Moringa oleifera leaves in two stages of maturity. Plant Foods for Human Nutrition, 64(4), 303–311. https://doi.org/10.1007/s11130-009-0141-0
Siddhuraju, P., & Becker, K. (2003). Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. Journal of Agricultural and Food Chemistry, 51(8), 2144–2155. https://doi.org/10.1021/jf020444+
Vergara-Jimenez, M., Almatrafi, M. M., & Fernandez, M. L. (2017). Bioactive components in Moringa oleifera leaves protect against chronic disease. Antioxidants, 6(4), 91. https://doi.org/10.3390/antiox6040091
Zhang, Y., Li, X., Wu, H., Liu, J., & Lu, Y. (2019). Phenolic profiles and antioxidant activity of Moringa oleifera leaves from different geographical locations. Molecules, 24(9), 1603. https://doi.org/10.3390/molecules24091603
Prior, R. L., Wu, X., & Schaich, K. (2005). Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry, 53(10), 4290–4302. https://doi.org/10.1021/jf0502698
Devi, S., Kumar, A., & Kumar, R. (2020). Moringa oleifera and its potential in food fortification and value-added product development: A review. International Journal of Food Science and Nutrition, 5(2), 45–52.
Unduhan
Diterbitkan
Terbitan
Bagian
Lisensi
I. ORIGINALITY, AUTHORSHIP, AND COPYRIGHT
The manuscripts submitted for publication must be research works written in English that have not been previously published and  are not being considered for publication elsewhere. All the authors must have agreed to the submission and to the order of their names on the title page. They must also have agreed that the corresponding author may act on their behalf throughout the editorial review and publication process. The corresponding author is responsible for obtaining such agreement.
Requests for changes in authorship (order of listing or addition or deletion of names) after submission should be accompanied by signed statements of agreement from all the parties involved. To maintain and protect the publisher‘s ownership and rights and to protect the original authors from misappropriations of their published work, author(s) are required to sign a Journal Publishing Agreement and send it to the editorial office (jhtm@uui.ac.id).
II. PERMISSION
The author is responsible for acquiring the permission(s) to reproduce any copyrighted figures, tables, data, or text that are being used in the submitted paper. Authors should note that text quotations of more than 250 words from a published or copyrighted work will require grant of permission from the original publisher to reprint. The written permission letter(s) must be submitted together with the manuscript.
III. EDITORIAL REVIEW AND REVISION
All papers will be critically read by at least two anonymous reviewers, selected for their competence in the subject area of the paper. Acceptance of the paper will depend upon its scientific merit and suitability for the journal. A paper may be accepted in its original form or subject to revision.
The reviewers' (and editor's) suggestions will be conveyed to the author, who will then have an opportunity to revise the paper. A manuscript returned to an author for revision can be held for a maximum of eight weeks.
The accepted paper will be edited by native English speakers. The cost of language editing service is currently free.
IV. PROOFS
Galley proofs (PDF format) for an accepted article will be sent by e-mail to the corresponding author for copyediting corrections. The core content of an article cannot be changed at this time. It is the author's responsibility to check the entire manuscript, including tables, figure legends, and cited reference numbers, not just the items queried. The proofs should be corrected and mailed to the editorial office within 48 hours of receipt in order to expedite publication. Only one set of corrections will be accepted. In other words, it is important to ensure that all of your corrections are sent to us in one communication.
V. Â REPRINTS
Five free reprint copies of the published article will be shipped to the corresponding authors in Indonesia only, approximately two weeks after the issue is printed. Authors, including overseas authors, who wish to purchase reprints, are required to complete the Reprint Order Form, which will be sent with the galley proofs to corresponding authors. This form must be returned together with the corrected galley proofs.
