PENETAPAN KADAR POLIFENOL EKSTRAK DAN FRAKSI KULIT PINANG (Areca catechu L.) DENGAN METODE SPEKTROFOTOMETRI UV-VIS

Authors

  • Yulianis Yulianis Stikes Harapan Ibu Jambi
  • Eka Fitriani Stikes Harapan Ibu Jambi
  • Mukhlis Sanuddin Stikes Harapan Ibu Jambi

Keywords:

Kata kunci, Kulit buah pinang, Polifenol, Spektrofotometri UV-Vis.

Abstract

Kulit buah pinang (Areca catechu L.) belum dimanfaatkan dengan baik sehingga sebagian besar masih menjadi limbah dan sampah organik. Kulit buah pinang memiliki kandungan senyawa polifenol. Penetapan kadar polifenol perlu dilakukan karena senyawa polifenol dapat berpotensi sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui kadar polifenol pada ekstrak dan fraksi kulit buah pinang. Kulit buah pinang diekstraksi secara maserasi menggunakan pelarut etanol 70%. Penetapan kadar polifenol total ditentukan dengan metode spektrofotometri UV-Vis menggunakan pereaksi Folin-Ciocalteu. Larutan kemudian diukur absorbansi dengan panjang gelombang 756 nm. Hasil penelitian didapat bahwa kadar polifenol total pada ekstrak sebesar 2,395485 %, fraksi n-heksan sebesar 0,100894 %, fraksi etil asetat sebesar 0,994891 %, fraksi n-Butanol sebesar 3,88974 % dan fraksi air sebesar 0,962963 %. Kadar senyawa polifenol terbesar pada sampel ekstrak dan fraksi n-Butanol kulit buah pinang.

 

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Published

2020-04-28

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Vol. 6 No. 1 (2020): APRIL 2020

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